Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel

Regeneration of periodontal tissues requires an integrated approach to the restoration ligament, cementum, and alveolar bone surrounding teeth. Current strategies in endogenous regenerative dentistry widely use biomaterials, particular decellularized extracellular matrix (dECM), facilitate recruitment populations resident cells into damaged stimulate their proliferation differentiation. The purpose our study was evaluate effect exogenous components (hyaluronic acid, laminin, fibronectin) on differentiation ligament stem (PDLSCs) cultured with dECM (combinations tooth matrices ligament) a 3D collagen I hydrogel. immunohistochemical expression various markers PDLSCs assessed quantitatively semi-quantitatively paraffin sections. results showed that under these conditions for 14 days exhibited phenotypic characteristics consistent osteoblast-like odontoblast-like cells. This potential has been demonstrated by osteogenic (OC, OPN, ALP) odontogenic (DSPP). phenomenon corresponds vivo state which at interface between cementum tend differentiate osteoblasts or cementoblasts. addition fibronectin most effectively induces culture conditions. Therefore, this bioengineered construct high future tissue regeneration.